Modifying Enzymes
Modifying Enzymes
Thermo Scientific™ RNase A, DNase and protease-free (10 mg/mL)
Specifically degrade single-stranded RNA at C and U residues with the endoribonuclease RNase A.
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Thermo Scientific™ DNase I, RNase-free (1 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
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Thermo Scientific™ Proteinase K, recombinant, PCR grade
Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.
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Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
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Pepsin, Extra Pure, Powder, SLR, Fisher Chemical™
Enzyme that breaks down proteins into smaller peptides.
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Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
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Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
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Invitrogen™ Ambion™ RNase A, affinity purified, 1 mg/mL
Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity
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Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
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Invitrogen™ Ribonuclease H
Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA
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Thermo Scientific™ RNase I (10 U/μL)
Ribonuclease I (RNase I) is an endoribonuclease that hydrolyzes single-stranded RNA to nucleoside 3'-monophosphates.
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