Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.

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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

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Specifications

Flow Rate	4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type	Pre-packed Column
Content And Storage	4°C to 30°C
Description	HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Material	Polypropylene
Support Type	6% Highly Cross-linked Agarose

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 M_r in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

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Thermo Scientific™ HisPur™ Ni-NTA Resin

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

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Specifications

Stationary Phase	Divalent Nickel, Metal Chelate
Type	Resin
Target	His-Tagged Protein
Content And Storage	Store at 4°C
Format	Bottle
Description	HisPur Ni-NTA Resin
Form	Liquid Suspension
Column Type	Affinity, Agarose Resin
Capacity (Metric)	Up to 60 mg of 6xHis-Tagged Protein Binding per mL Resin
Product Line	HisPur™

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

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Invitrogen™ Ni-NTA Agarose

Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence

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Specifications

Type	Agarose
Stationary Phase	Ni-NTA
Content And Storage	The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.
Form	Suspension
Column Type	Affinity Column

IBA Lifesciences Strep-Tactin™XT 4Flow™ High Capacity Resin

Strep-Tactin™XT 4Flow™ high capacity resin is a 50% suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

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Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 3 mL

Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.

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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents

Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

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Specifications

Buffer	Lysis Buffer
Shipping Condition	Ambient
Form	Liquid
Product Type	Nuclear and Cytoplasmic Extraction Reagent
Reagent Type	Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Product Line	NE-PER™

Thermo Scientific™ CaptureSelect™ IgG4 (Hu) Affinity Matrix

Thermo Scientific™ CaptureSelect™ IgG4 (Hu) Affinity Matrix has been specifically designed for the purification and isolation of human IgG4 from complex sources such as plasma, serum, or cell culture supernatants.

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Specifications

Particle Size	65 ±10 μm
Ligand Type	IgG4 affinity ligand
Flow Rate	50 to 200 cm/hr. (Up to 2 bar)
Type	Affinity Matrix
Matrix	Agarose-based, epoxide activated
Form	Resin Slurry
Binding Capacity	>15 g IgG4/L resin
Product Line	CaptureSelect™

IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension

Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

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Specifications

Particle Size	50 to 150 μm
Ligand Type	Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Stationary Phase	Agarose
Type	Bulk Resin
Target	Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin
Format	Bottle
Description	4% agarose, crosslinked
Final Product Type	Affinity chromatography equipment
Protein Form	Tetrameric protein
Form	Resin Slurry
Binding Capacity	20 mg/mL resin
Column Type	Suspension
Concentration	50% suspension

IBA Lifesciences Strep-Tactin™ 4Flow™ High Capacity Cartridge

Strep-Tactin™ 4Flow™ high capacity cartridge is a HPLC/FPLC cartridge to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins

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Specifications

Particle Size	50 to 150 μm
Target	Twin-Strep-Tag™ and Strep-tag™II
Format	Pre-packed Cartridge
For Use With (Application)	HPLC/FPLC
Support Type	4% agarose, crosslinked

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

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Specifications

Flow Rate	<20 mL/min.
Type	Pre-packed Column
Content And Storage	4°C to 30°C, 20% Ethanol
Diameter (English) Inner	0.6 in.
Description	HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure	5 bar (0.5 MPa, 70 psi)
pH Range	3 to 12
Diameter (Metric) Inner	16 mm
Material	Polypropylene
Support Type	6% Cross-linked Agarose