Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ HindIII (10 U/μL)

The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

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Specifications

Concentration	10 U/μL

Thermo Scientific™ FastDigest NcoI

The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Nco I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	SacI
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	3' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest DpnI

Cut at Gm6A^TC sites with Thermo Scientific™ FastDigest™ DpnI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MalI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Dpn I
Methylation Sensitivity	Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	Blunt
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest NotI

The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Not I
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	80°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest XbaI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Xba I
Methylation Sensitivity	Dam Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest BcuI

FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Bcu I
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest PacI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Eco31I
Methylation Sensitivity	CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest MluI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

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Thermo Scientific™ FastDigest BglII

Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	Bgl II
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

Thermo Scientific™ FastDigest BshTI

Cut at A^CCGGT sites with Thermo Scientific™ FastDigest™ AgeI (BshTI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.

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Thermo Scientific™ BsuRI (HaeIII) (10 U/μL)

Cut at GG^CC sites with BsuRI (HaeIII) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: BshFI, BsnI, BspANI, HaeIII, PhoI).

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Thermo Scientific™ FastDigest MunI

Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.

Promotions are available

Pricing and Availability
Specifications

Sensitive to Heat Inactivation	No
Product Type	Restriction Enzyme
Enzyme	MunI
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Overhang	5' protruding ends
Type IIS RE	No
Optimal Reaction Temperature	37°C
Product Line	FastDigest™

Thermo Scientific™ BpiI (BbsI) (10 U/μL)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

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Pricing and Availability
Specifications

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BpiI (BbsI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentration	10 U/μL

Restriction Enzymes

Restriction Enzymes

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