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Fisher Scientific
Products
Biochemical Reagents
DNA Reagents
PCR Cloning Kits
PCR Cloning Kits
PCR Cloning Kits
Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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Biochemical Reagents
DNA Reagents
PCR Cloning Kits
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Invitrogen
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Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with One Shot™ TOP10 chemically competent
E. coli
cells
For fast, efficient cloning and subsequent in vitro transcription/translation
Pricing and Availability
Specifications
Promoter
T7, SP6
Content And Storage
Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™
E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.
Store at -68 to -85°C.
Format
Kit
Product Type
Cloning Kit
Cloning Method
TOPO™-TA
Bacterial or Yeast Strain
TOP10
Cell Type
Chemically Competent