Optimize Your Immunoprecipitation (IP) with ChromoTek Nano-Traps
Ready-to-Use Affinity Beads for Fast and Efficient Immunoprecipitation
ChromoTek Nano-Traps are the benchmark in immunoprecipitation (IP) and allow fast and reliable single-band pulldown of low expressed proteins. They consist of nanobodies coupled to beads and are ready to use in the following applications: IP, Co-IP, Co-IP/mass spectrometry (MS), on-bead assay, ChIP/RIP analysis, and split-fluorescent protein assay.
Browse all Chromotek Nano-Traps
Browse all Chromotek Nanobody-based Reagents
Nano-Trap Benefits
The benefiits of the Nano-Trap system include:
- No heavy and light antibody chains
- Stringent washing conditions
- Low host-cell protein background
- Very efficient pulldown, with no protein in flow-through
- High affinity to binding of proteins expressed at low levels
- Short incubation time (30-60 min)
- Recombinantly expressed and validated
Available Nano-Trap Targets
Examples of Nano-Trap targets include those listed below and many more:
- GFP
- V5
- DYKDDDDK
- Myc
- HA-tag
- Ubiquitin
When using Nano-Traps, the amount of immunoprecipitated protein of interest (POI) is significantly higher and the background is reduced in contrast to an IP conducted with a conventional antibody (See Figure 1). Nano-Traps provide pure fractions of immunoprecipitated POI without contamination of heavy and light antibody chains.
Figure 1: IP of a protein of interest (POI) by Nano-Traps (left) compared with conventional antibodies coupled to Protein A/G beads (right), analyzed by SDS-PAGE. M: marker; B: bound.
GFP-Traps for Immunoprecipitation
When using a GFP-Trap™ for pull-down of GFP-fusion proteins, the amount of immunoprecipitated GFP is significantly higher and the background is reduced in contrast to an IP conducted with a conventional anti-GFP antibody conjugated to Protein A/G beads.
No Heavy and Light Chain Antibody Fragments, and Superior Background
Heavy and light antibody chains are contaminating; however, a GFP-Trap provides pure fractions of immunoprecipitated GFP-fusion protein without such contamination (see Figure 2). ChromoTek GFP-Traps have been cited by researchers over 5,000 times and are the most frequently cited monoclonal anti-GFP antibody. As such, they are the gold standard for immunoprecipitation of GFP-fusion protein.
Figure 2: IP of GFP using a GFP-Trap compared with a conventional anti-GFP antibody coupled to Protein A/G beads, analyzed by SDS-PAGE. B: bound; I: input; FT: flow-through.
Ready-to-Use GFP-Trap Formats
GFP-Traps consists of an anti-GFP nanobody conjugated to different resins. The GFP-Trap is available as agarose beads, magnetic agarose beads, magnetic particles, or 96-well plates. Examples of these GFP-Traps and their features/uses are given below:
- GFP-Trap Agarose: Lowest background and high binding capacity IP
- GFP-Trap Magnetic Agarose: Magnetic separation and high binding capacity IP
- GFP-Trap Magnetic Particles (M-270): Pull-down of large proteins/complexes
- GFP-Trap Multiwell Plates: High-throughput applications and ELISA
- GFP-Trap Kits: Including lysis buffer for mammalian cells, wash, and elution buffers
