Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

1-Step™ Chloronaphthol Substrate Solution

Brand:  Thermo Scientific™ 34012

Product Code. 11809260

Description

Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.

Features of Chloronaphthol Substrates:

HRP substrate—for detection of horseradish peroxidase activity on solid media

Chromogenic—no special equipment needed for visualization

Blue-purple—easy to photograph in color or black-and-white

Package options—choose powder or ready-to-use solution

Highlights of Pierce CN Solution

Ready-to-use—no ethanol or methanol required to dissolve; no dilution necessary for use

Complete—does not require buffer or addition of hydrogen peroxide

Pre-filtered—does not need to be filtered before use like powders or tablets after they are dissolved

Stable—store refrigerated at 4°C for at least one year

Specifications

• Product Type: Substrate
• Form: Liquid
• Quantity: 250 mL
• Reactivity: Horseradish Peroxidase

• Detection Method: Colorimetric
• Product Line: 1-Step™
• Substrate: Horseradish Peroxidase
• Content And Storage: Store at 4°C.

Safety and Handling

Product Identifier

• 1-Step Chloronaphthol Substrate Solution

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Specific target organ toxicity Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H371-May cause damage to organs.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol

For Research Use Only. Not for use in diagnostic procedures.