Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

Brand:  Thermo Scientific™ 34018

Product Code. 10066433

Description

Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.

TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.

Highlights:

• HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media

• Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates

• Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use

• Complete – does not require other buffers or addition of hydrogen peroxide

Specifications

• Product Type: Substrate
• Form: Liquid
• Quantity: 250 mL
• Reactivity: Horseradish Peroxidase

• Detection Method: Colorimetric
• Product Line: 1-Step™
• Substrate: Horseradish Peroxidase
• Content And Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Safety and Handling

Product Identifier

• 1-Step TMB-Blotting, 250 mL

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Specific target organ toxicity Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H371-May cause damage to organs.

Precautionary Statement

• P270-Do not eat, drink or smoke when using this product.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.
• P308+P311-IF exposed or concerned: Call a POISON CENTER/doctor/.

Supplemental information

• MIXTURE LIST-Contains : Methyl alcohol

For Research Use Only. Not for use in diagnostic procedures.