3,3',5,5'-Tetramethylbenzidine, 98%, Thermo Scientific Chemicals

Description

3,3',5,5'-Tetramethylbenzidine, 98%, or TMB, CAS # 54827-17-7, is a colorimetric reagent used in laboratory-based substrate detection.

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3',5,5'-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• 3,3',5,5'-Tetramethylbenzidine, also known as 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl or TMB, is a colorimetric substrate for detection of horseradish peroxidase-labeled probes

• Oxidation of 3,3',5,5'-Tetramethylbenzidine results in a pale blue end product that can be detected at 370 or 655 nm. Sulfuric acid (H2SO4) is used to stop the oxidation reaction and stabilize the reading, resulting in a yellow color that can be read at 450 nm

Application

• 3,3',5,5'-Tetramethylbenzidine is used as a chromogenic visualization agent in enzyme-linked immunosorbent assays (ELISA) or immunohistochemistry (IHC) techniques

• 3,3',5,5'-Tetramethylbenzidine has been used to detect molecules in aqueous samples such as copper ion and hypochlorite

Specifications

Chemical Identifiers

• CAS: 54827-17-7
• Molecular Formula: C16H20N2
• Molecular Weight (g/mol): 240.35
• MDL Number: MFCD00007748
• InChI Key: UAIUNKRWKOVEES-UHFFFAOYSA-N
• Synonym: 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
• PubChem CID: 41206
• IUPAC Name: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

• Melting Point: 168°C to 171°C
• Quantity: 1 g
• Beilstein: 2808541
• Sensitivity: Light and moisture sensitive
• Solubility Information: Soluble in ethyl acetate,ethanol,acetone,chloroform,toluene and dimethyl sulfoxide. Insoluble in water.
• Formula Weight: 240.35
• Percent Purity: 98%
• Chemical Name or Material: 3,3',5,5'-Tetramethylbenzidine

Safety and Handling

Product Identifier

• 3,3',5,5'-Tetramethylbenzidine

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Germ cell mutagenicity Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.
• H335-May cause respiratory irritation.
• H341-Suspected of causing genetic defects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.

Supplemental information

• MIXTURE LIST-Contains : 3,3',5,5'-tetramethyl-[1,1'-Biphenyl]-4,4'-diamine

RUO – Research Use Only