3,3',5,5'-Tetramethylbenzidine aq. soln., Thermo Scientific™

Description

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Applications
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays. 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood assay of hemoglobin assay of peroxidases.

Solubility
Slightly soluble. <0.1 g/100 mL at 20°C.

Notes
Contains TMB in an aqueous buffer, pH 3.6 ± 0.2, with 0.03% hydrogen peroxide. The TMB product is soluble after conversion

Specifications

Chemical Identifiers

• CAS: 54827-17-7
• Molecular Formula: C16H20N2
• Molecular Weight (g/mol): 240.35
• MDL Number: MFCD00007748
• InChI Key: UAIUNKRWKOVEES-UHFFFAOYSA-N
• Synonym: 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
• PubChem CID: 41206
• IUPAC Name: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

• Color: Yellow
• Quantity: 1000 mL
• Beilstein: 2808541
• Formula Weight: 240.35
• Physical Form: Liquid
• Chemical Name or Material: 3,3',5,5'-Tetramethylbenzidine