4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester, 98+%, Thermo Scientific Chemicals

Description

4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. It is also used for N-terminal modification of peptides in automated synthesis.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester is a useful reagent (similar to MANCYL-SE) for labeling proteins or peptides through their amino-groups by forming stable peptide bonds. The succinimidyl ester is reactive with terminal amines or lysines of peptides and other nucleophiles for fluorescent studies of proteins. Its fluorescent properties (has a characteristic broad and intense visible absorption but has no fluorescence) make it an ideal long wavelength quencher and it has been utilized as an acceptor chromophore in FRET studies. It is also used for N-terminal modification of peptides in automated synthesis.

Solubility
Soluble in DMSO.

Notes
Moisture sensitive. Incompatible with oxidizing agents and heat.

Specifications

Chemical Identifiers

• CAS: 146998-31-4
• Molecular Formula: C19H18N4O4
• Molecular Weight (g/mol): 366.377
• MDL Number: MFCD00467415
• InChI Key: IBOVDNBDQHYNJI-UHFFFAOYSA-N
• Synonym: 2,5-dioxopyrrolidin-1-yl 4-4-dimethylamino phenyl diazenyl benzoate, dabcyl se dye, dabcyl acid, se, 4-4-dimethylamino phenyl azo benzoic acid, succinimidyl ester, 4-4-dimethylamino phenylazo benzoic acid n-succinimidyl ester, dabcyl-nhs, e-2,5-dioxopyrrolidin-1-yl 4-4-dimethylamino phenyl diazenyl benzoate, 2,5-pyrrolidinedione, 1-4-4-dimethylamino phenyl azo benzoyl oxy, dabcyl-se; 4-4-dimethylamino phenylazo benzoic acid n-succinimidyl ester, dabcyl se, solid
• PubChem CID: 4206604
• ChEBI: CHEBI:52005
• IUPAC Name: (2,5-dioxopyrrolidin-1-yl) 4-[[4-(dimethylamino)phenyl]diazenyl]benzoate
• SMILES: CN(C)C1=CC=C(C=C1)N=NC2=CC=C(C=C2)C(=O)ON3C(=O)CCC3=O

• Quantity: 25 mg
• Sensitivity: Moisture sensitive
• Solubility Information: Soluble in DMSO.
• Formula Weight: 366.37
• Percent Purity: ≥98%
• Chemical Name or Material: 4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester

RUO – Research Use Only