5-hydroxymethylcytosine Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA540097

Product Code. 15596781

Description

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

Specifications

• Antigen: 5-hydroxymethylcytosine
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: 5hmc; 5-hmc; 5-HydroxymethylCytidine; C5H7N3O2
• Immunogen: Human KLH-conjugated 5-hydroxymethylcytosine.
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C or -80°C if preferred
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Peptide Array
• Concentration: 3.5 mg/mL
• Formulation: PBS with 0.05% ProClin 300, 0.05% sodium azide; pH 7.4
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG