Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA) Promotion

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Brand:  Thermo Scientific™ B49

Product Code. 15707496

Description

Use the Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Usage Recommendations

• Use fresh 1X TAE both for the gel and for the electrophoresis run
• To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

• 40mM Tris
• 20mM acetic acid
• 1mM EDTA
• pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.

Specifications

• Components: 40mM Tris; 20mM acetic acid; 1mM EDTA; pHof 50X TAE: 8.4
• Content And Storage: There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.
• Quantity: 1 L
• Product Type: 50X TAE Buffer (Tris-acetate-EDTA)