Thermo Scientific™ ABsolute QPCR Mix, SYBR Green, no ROX

Optimized for SYBR Green chemistry and contains all the components necessary to perform quantitative PCR, with the exception of template and primers.

Product Code	Brand	No. of Reactions
11827692	Thermo Scientific™ AB1158B	1,600 Reactions

Description

The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

• Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers
• The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets
• ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, low ROX concentration, fluorescein or no reference dye
• A master mix optimized for capillary instruments is also available

Highlights

• High Sensitivity & Specificity – The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
• Optimised buffer containing SYBR Green I
• Reproducibility – All master mixes undergo extensive QC to ensure batch to batch consistency
• Store at -20°C

Specifications

• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Standard
• Product Line: ABsolute
• For Use With (Application): Gene Expression
• Concentration: 2X

• GC-Rich PCR Performance: High
• Polymerase: Taq DNA Polymerase
• For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
• Product Type: Real Time PCR SYBR Master Mix
• Compatibility: qPCR Cyclers
• Sample Type: DNA, dsDNA

For Research Use Only. Not for use in diagnostic procedures.