Thermo Scientific™ aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

Brand:  Thermo Scientific™ K1261

Product Code. 11901421

Description

aLICator LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in E. coli. The pLATE bacterial expression vectors are designed for high levels of target protein expression in concert with minimal background (uninduced) expression, which permits expression of proteins that are toxic to E. coli cells. To streamline and facilitate the process of insert cloning into the expression vector, the aLICator system uses directional LIC cloning technology, a rapid procedure that provides high-cloning efficiencies.

The tightly regulated expression and fast, efficient directional cloning makes the aLICator LIC Cloning and Expression System the best choice for routine and toxic gene cloning and expression in E. coli.

Highlights

• High efficiency LIC cloning
• Tight control of gene expression
• High yield expression
• Versatile -tagged or untaged protein expression with tag removal option

Specifications

• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Format: Kit
• For Use With (Application): PCR Cloning
• Product Line: aLICator
• Promoter: T7
• Quantity: 20 Reactions

• Cloning Method: LIC Cloning
• Sample Type: PCR Products
• No. of Reactions: 20 Reactions
• Product Type: LIC Cloning and Expression Kit
• Protein Tag: His Tag (6x)
• Vector: pLATE

Safety and Handling

Product Identifier

• aLICator LIC Cloning and Expression Kit 3 (C-terminal His-tag)

Signal Word

• Warning

Hazard Category

• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H373-May cause damage to organs.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P314-Get medical advice/attention if you feel unwell.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Glycine, N,N-1,2-ethanediylbis[N-(carboxymeth yl)-, disodiumsalt, dihydrate

For Research Use Only. Not for use in diagnostic procedures.