alpha Tubulin Monoclonal Antibody (TU-01), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA119162

Product Code. 11554532

Description

This antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin. HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins). Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay. Western Blot: Reducing conditions.

Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

Specifications

• Antigen: alpha Tubulin
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 15mM sodium azide; pH 7.4
• Gene Accession No.: A6NHL2, P02550, P05213, P0DPH7, P68363, P68366, P68368, P68369, P68370, P68373, Q2XVP4, Q3KRE8, Q3UX10, Q5XIF6, Q6AYZ1, Q6P9V9, Q6PEY2, Q71U36, Q9BQE3, Q9BVA1, Q9CWF2
• Gene Symbols: Tuba1a, Tuba1b, TUBA1C, TUBA3C, TUBA3E, TUBA4A, TUBAL3, TUBB2B
• Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Multi-species, Mouse, Porcine, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: TU-01
• Conjugate: Unconjugated
• Gene: TUBA4A
• Gene Alias: &agr-tubulin 84B; 1t; a1t; ALPHA 84C; alpha tubulin; alpha tubulin 84B; alpha[[1]] tubulin; alpha[[1]]-tubulin; alpha1; alpha1 tubulin; alpha1t; alpha1tub; alpha1tub84B; alpha1-tubulin; alpha84B; alphaT; alphat-1; alphatub; alpha-tub; alphaTub1; alphatub84; alphaTUB84B; alpha-tub84B; alphaTub84B-PA; alphatubulin; alpha-tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; Alpha-tubulin 3E; alpha-tubulin 4; Alpha-tubulin 6; alphaTubulin 84B; alpha-Tubulin 84B; alpha-Tubulin at 84B; alpha-tubulin I; Alpha-tubulin II; alpha-tubulin III; Alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin TUB1; alpha-tubulin ubiquitous; alphaTubulin84B; Alpha-Tubulin84B; ALS22; anon-EST:Liang-1.59; anon-EST:Liang-2.30; AT25469p; aTub84B; a-tub84B; bA408E5.3; B-ALPHA-1; bcm948; BEST:LD32507; cb944; CG1913; CG1913-PA; chr3R:2914276..2914416; clone 1.59; c
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 100135051, 100151951, 100153507, 100623355, 10376, 112714, 22142, 22143, 22145, 22146, 238463, 291081, 291287, 300218, 316531, 347733, 500929, 64158, 7277, 7278, 733594, 73710, 7846, 79861, 84790
• Content And Storage: 4° C, do not freeze
• Form: Liquid