Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Brand:  Invitrogen™ A25602

Product Code. 15460984

Description

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

This new addition to the family includes a TaqMan™ 1-Step RT-PCR master mix. The single-tube format of the RT-PCR step streamlines the 'sample to answer' workflow more than ever. Combining the simple Cells-to- CT™ lysis technology with the fast cycling capability of the 1-step RT-PCR master mix allows quicker time to results with limited sample handling.

The Cells-to-CT™ 1-Step TaqMan™ Kit is:

• Complete—optimized workflow includes cell lysis reagents with gDNA removal and TaqMan™ 1-Step RT-PCR Master Mix
• Faster than ever—7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for ∼35 minute sample-to-answer workflow
• Simple—10 steps total (sample prep and real-time RT-PCR combined)
• Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA
• Convenient—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs

Note: TaqMan™ primer/probe sets are sold separately.

Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR

Whether you are using plates or tubes, the Cells-to-CT™ 1-Step TaqMan™ Kit uses a simple 7-minute sample preparation procedure. The lysis technology is designed for 10–100,000 cultured cells/sample. Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment. Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution. The lysate is now ready to analyze on your real-time PCR instrument. Simply add it to a real-time RT-PCR master mix containing the TaqMan™ primer/probe sets of interest. Alternatively, the lysate can be stored at –20°C. Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing. Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required. The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes.

A Complete, Validated Solution

All components of the Cells-to-CT™ 1-Step TaqMan™ Kit have been optimized for consistent and reliable performance. This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes. For added quality assurance, the Cells-to-CT™ 1-Step TaqMan™ Kit has been validated with TaqMan™ Gene Expression assays and shows performance equivalent to that obtained with purified RNA.

Accessory Product

The TaqMan™ Cells-to-CT™ Control Kit (Cat. No. 4386995) was designed for use with TaqMan™ Cells-to-CT™ products. This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan™ Gene Expression assay to monitor the effects of PCR inhibition.

Specifications

• Content And Storage: Lysis Solution, 22 mL, store at 2°C to 8°C, DNase I, 220 μL, store at -5 to -30°C, Stop Solution, 2 x 1 mL, store at -5 to -30°C, TaqMan™ 1-step qRT-PCR Mix, 10 mL, store at -5 to -30°C,
• Format: Tube
• PCR Method: 1-step RT-qPCR
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• Product Line: Cells-to-CT™, TaqMan™
• Quantity: 400 preps
• No. of Reactions: 400 Reactions
• Sample Type: Mammalian Cells
• Volume: 22 mL

• Detection Method: TaqMan
• GC-Rich PCR Performance: High
• Reaction Speed: 7 min.
• Green Features: Fewer resources used and less waste, Less hazardous
• Product Type: 1-Step qPCR kit
• For Use With (Application): Gene Expression
• Reagent Type: Cell Lysis
• Starting Material: 10-100,000 Cells

Safety and Handling

Product Identifier

• Cells-to-CT 1-Step TaqMan Kit

Signal Word

• Warning

Hazard Category

• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P264-Wash thoroughly after handling.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Glycerin, Ethylene oxide (impurity), Glycerin, Dimethyl sulfoxide, Ethylene glycol, Tris (hydroxymet

For Research Use Only. Not for use in diagnostic procedures.