Ambion™ Exo-Klenow Fragment (cloned), DNA Polymerase I, 5U/μL

No exonuclease activity

Brand:  Invitrogen AM2008

Product Code. 10196904

Includes: One tube containing 750U (5U/μL)

Description

• Ambion Exonuclease-free Klenow Fragment is a genetically engineered Klenow polymerase to remove 3' to 5' exonuclease activity has been removed, leaving only the 5' to 3' polymerase activity
• One tube containing 750U (5U/μL) is provided
• The elimination of exonuclease activity makes Exo-Klenow Fragment the enzyme of choice for isotopic or biotin labeling of DNA probes by the random-primed method, and for DNA sequencing by the Sanger dideoxy method. The Exo-Klenow Fragment is thoroughly tested for nonspecific contaminating endonuclease, exonuclease, and protease activity
• Functionality is determined with a random-priming reaction
• Unit Definition: One unit of enzyme incorporates 10nmol of deoxynucleotides into acid insoluble material in 30 minutes at 37°C

Cloning, Restriction Enzyme Cloning

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: One tube containing 750U (5U/μL). Store at -20deg.C
• Quantity: 750 units
• For Use With (Application): DNA Sequencing
• Concentration: 5U/μL

For Research Use Only. Not for use in diagnostic procedures.