Invitrogen™ FirstChoice™ RLM-RACE Kit

Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR

Includes: Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5M EDTA should be stored at –20°C. Nuclease-free water may be stored at any temperature.

Product Code	Brand	Includes
10066184	Invitrogen™ AM1700M	Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature and manual
10554445	Invitrogen™ AM1700	Exo– Klenow, 10X Decamer Solution, 5X Reaction Buffer minus dATP, 5X Reaction Buffer minus dCTP, DECAtemplate GAPDH-M, and 0.5 M EDTA should be stored at -20°C. Nuclease-free water may be stored at any temperature.

Description

The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol. The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages.

• From RNA to PCR product in less than a day
• Yields single, specific product from rare transcripts
• Selects 5' and/or 3' ends of true messages
• Efficient—
• Al enzymatic reactions are optimized to ensure detection of even rarest mRNA
• Final product size: 7kb or less
• Optimal reaction temperature: 42°C
• Sample: RNA

• Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages
• Improvement to basic RACE protocol
• Selects full-length mRNA—no rRNA, tRNA or degraded mRNA

Optimal reaction temperature: 42°C

Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

Order Info

Shipping Condition: Dry ice

Specifications

• Product Type: RLM-RACE Kit
• Optimal Reaction Temperature: 42°C
• Format: Kit
• For Use With (Application): cDNA Libraries & Library Construction

• Final Product Type: cDNA
• Reverse Transcriptase: M-MLV
• Product Line: Ambion™, FirstChoice™
• Quantity: 1 Kit

For Research Use Only. Not for use in diagnostic procedures.