Invitrogen™ Ambion™ In Vivo Negative Control #1 siRNA

Ambion™ In Vivo Negative Control #1 siRNA

Product Code	Brand	Quantity
15521104	Invitrogen™ 4457287	5 nmol
15561114	Invitrogen™ 4459405	50 nmol
15541104	Invitrogen™ 4457289	250 nmol

Description

Ambion™ In Vivo siRNA Controls

Validated siRNA controls for optimizing siRNA experiments targeting coding and noncoding transcripts

Positive Control siRNAs functionally tested in several common cell lines

Negative Controls functionally proven to have minimal effects on cell proliferation and viability

Include Silencer™ Select modifications for enhanced specificity or Ambion™ In Vivo modifications for increased stability

For use in human, mouse, and rat cells

What are Ambion™ In Vivo siRNAs?
Ambion™ In Vivo siRNAs are designed using the proven Silencer™ Select algorithm and incorporate chemical modifications for superior serum stability with in vivo delivery. The added serum stability does not compromise the performance of the siRNAs. Ambion™ In Vivo siRNAs have been shown to be non-toxic in vivo (mouse) and non-immunogenic (cell-based assays). In cell-based assays, Ambion™ In Vivo siRNAs exhibit equivalent or better potency than Silencer™ Select siRNAs. The result is better confidence in your in vivo RNAi experiments, better knockdown when paired with an efficient delivery solution, and the assurance of limited non-specific effects.

Negative Control siRNAs
Negative control siRNAs—siRNAs with sequences that do not target any gene product—are essential for determining the effects of siRNA delivery and for providing a baseline to compare siRNA-treated samples. Ambion™ In Vivo siRNAs and have no significant sequence similarity to mouse, rat, or human gene sequences. These negative control siRNAs have been tested by microarray analysis and shown to have minimal effects on gene expression. In addition, these negative control siRNA has been tested in multi-parametric cell-based assays and are proven to have no significant effect on cell proliferation, viability, or morphology in the cell lines tested.

Quality Control
Ambion™ synthesizes and purifies each Ambion™ In Vivo siRNA in state-of-the-art facilities to meet the highest quality standards. As part of our rigorous quality control procedures, each RNA oligonucleotide is analyzed by MALDI-TOF mass spectrometry, and analytical HPLC is used to monitor purity. To provide the utmost in quality, we also assess each annealed siRNA by gel electrophoresis to confirm that the strands anneal properly. The result is premium-quality siRNA that is purified and ready to use.

In Vivo Ready Controls
Our In Vivo Ready Control siRNAs are subjected to that extra level of purification and testing required for the introduction of siRNAs into animals. After HPLC purification and annealing, each siRNA is further purified utilizing a process that removes excess salt via a semi-permeable membrane. The result is highly pure siRNA with minimal salt content, suitable for in vivo applications. In vivo siRNAs are then sterile filtered, and tested for the presence of endotoxin. At concentrations of 50μM in presence of deionized water, in vivo Ready siRNAs contain less than 5.0 mM Na+, less than 0.06 mM K+, and less than 0.02 mM Mg2+.

Specifications

• Content And Storage: The siRNA (annealed) is shipped at ambient temperature as dried down oligonucleotides in a single tube, along with 1.75 ml Nuclease-free Water for resuspension. Store at or below –20°C.
• RNAi Type: siRNA
• Label or Dye: Unconjugated
• Product Type: siRNA
• Shipping Condition: Room Temperature

• Control Type: Negative Control
• For Use With (Application): RNAi, Transfection
• Product Line: Ambion™
• Purity: In Vivo, HPLC

For Research Use Only. Not for use in diagnostic procedures.