Invitrogen™ mirVana™ miRNA Isolation Kit, with phenol

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method.

Brand:  Invitrogen™ AM1560

Product Code. 10763147

Description

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.

Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 10²–10⁷ cultured cells or 0.5–250 mg tissue.

Enrich for small RNA
Although the mirVana miRNA Isolation Kit efficiently purifies all RNA larger than 10nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana miRNA Isolation Kit, RNA molecules of ∽200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Accessory products
For tissues that have very high levels of ribonucleases, such as pancreas or spleen, the mirVana miRNA Isolation Kit is recommended, although it is not designed to recover protein and small RNA from the same sample.

Not high-throughput compatible (Manual)

Specifications

• Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)
  • 50 mL Wash Solution 2/3 (room temperature)
  • 80 Collection Tubes (room temperature)
  • 40 Filter Cartridges (room temperature)
  • 100 mL Lysis/Binding Buffer (4°C)
  • 10 mL miRNA Homogenate Additive (4°C)
  • 100 mL Acid-Phenol:Chloroform (4°C)
  • 1.4 mL Gel Loading Buffer II (-20°C)
  • 5 mL Elution Solution (-20°C, 4°C, or room temperature)
• Purification Time: 30 min. (After sample homogenization)
• Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 40 Preps
• Starting Material Amount: Bacteria: ≤10⁷ cells
  Cells: ≤10⁷
  Plant: ≤250 mg
  Tissue: ≤250 mg
  Virus: ≤10⁷ cells
  Yeast: ≤10⁷ cells
  Enzymatic Reactions: varies

• Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
• Elution Volume: 100 μL
• Final Product Type: Small RNA
• For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
• Shipping Condition: Room Temperature
• Yield: Cells: ≤16 μg RNA per 50,000 cells
  Tissue: ≤160 μg per 5 mg

Safety and Handling

Product Identifier

• mirVana miRNA Isolation Kit, with phenol

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Germ cell mutagenicity Category 2
• Reproductive toxicity Category 1B
• Reproductive toxicity Category 2
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 1 C
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity Category 1
• Specific target organ toxicity after repeated exposure Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H301-Toxic if swallowed.
• H302-Harmful if swallowed.
• H311-Toxic in contact with skin.
• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H331-Toxic if inhaled.
• H335-May cause respiratory irritation.
• H341-Suspected of causing genetic defects.
• H351-Suspected of causing cancer.
• H360-May damage fertility or the unborn child.
• H361-Suspected of damaging fertility or the unborn child.
• H372-Causes damage to organs.
• H373-May cause damage to organs.
• H411-Toxic to aquatic life with long lasting effects.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P272-Contaminated work clothing should not be allowed out of the workplace.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P314-Get medical advice/attention if you feel unwell.
• P330-Rinse mouth.
• P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : phenol, chloroform,2-Mercaptoethanol, Thiocyanic acid, Formamide

For Research Use Only. Not for use in diagnostic procedures.