Invitrogen™ Power SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Power SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Brand:  Invitrogen™ 4402954

Product Code. 10249494

Description

Ambion Power SYBRGreen Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. Power SYBRGreen Cells-to-C_T Kit is Powered by AmpliTaq Gold™ DNA Polymerase, LD, which provides the highest levels of specificity with standard real-time PCR.

• Includes optimized reagents to work efficiently, right out of the box
• Simple, effective Cells-to-C_T technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment
• Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNA

Extraordinary Value
The kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBRGreen.

Extraordinary Ease
The protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at -20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.

Extraordinary Performance
To demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the Power SYBRGreen Cells-to-C_T Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems, Roche, and Bio-Rad.

• PCR Method: 2-Step qRT-PCR
• Purity/Quality Grade: LD (Low DAN)
• Passive Reference Dye: ROX (Pre-mixed)
• Polymerase: AmpliTaq Gold™ DNA Polymerase

Accessory Product:
The SYBRGreen Cells-to-C_T Control Kit (Cat. No. 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-C_T reactions.

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, and Power SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
• Polymerase: AmpliTaq Gold DNA Polymerase
• For Use With (Equipment): 7000 System, 7200 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 5700, GeneAmp 9600, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
• Label or Dye: SYBR Green
• Product Line: Cells-to-CT™, SYBR™
• Purity or Quality Grade: LD (Low DNA)
• Shipping Condition: Dry Ice
• Concentration: 20X
• No. of Reactions: 100 reactions

• Detection Method: SYBR
• Reaction Speed: Standard
• Green Features: Fewer resources used and less waste, Less hazardous
• Passive Reference Dye: ROX (Pre-mixed)
• Purification Time: 5 min.
• Reverse Transcriptase: M-MLV
• For Use With (Application): RT-PCR
• Hot Start: Built-In Hot Start
• Sample Type: Cells

For Research Use Only. Not for use in diagnostic procedures.