Invitrogen™ Ambion™ MessageSensor™ RT Kit

For the reverse transcription of RNA

Brand:  Invitrogen™ AM1745

Product Code. 10792687

Includes: 10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, hGAPDH Control Primers, HeLa S3 Control RNA, and nuclease-free water

Description

The Ambion kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions.

• Linear quantitation of mRNA targets over 10⁶-fold range of input RNA
• Quantitate transcripts from as little as 500fg total RNA—1/20th of a single cell
• Fast, flexible RT step complete in 15 min.; use reaction temperatures up to 50°C
• Compatible with TaqMan™, SYBR™ Green, and gel-based detection methods
• Optimal reaction temperature: 42° to 50°C
• Final product size: 7kb or less

MessageSensor is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity. The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than two hours.

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: 10X RT-PCR Buffer, dNTP Mix, RNase Inhibitor, M-MLV RT, 10X Glycerol, hGAPDH Control Primers, HeLa S3 Control RNA, and nuclease-free water
• Quantity: 50 reactions
• Sample Type: RNA

• Format: Separate Components
• For Use With (Application): cDNA Synthesis, RT-PCR

For Research Use Only. Not for use in diagnostic procedures.