Invitrogen™ pMIR-REPORT™ miRNA Expression Reporter Vector System

For the measurement of miRNA expression in cells

Brand:  Invitrogen™ AM5795

Product Code. 10321045

Description

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR miRNA Inhibitor Molecules, respectively.

• Contains two mammalian expression vectors: one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency
• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

RNAi, RNAi, Epigenetics and Non-Coding RNA Research, Vector-Based RNAi, miRNA and Non-Coding RNA Analysis, miRNA Functional Analysis

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: RNAi Expression Vector Kit
• Content And Storage: pMIR-REPORT™ Luciferase and pMIR-REPORT™ β-gal Control Plasmid are both supplied as glycerol stocks in E. coli and should be stored at –70°C.
• Constitutive or Inducible System: Constitutive
• RNAi Type: miRNA
• Shipping Condition: Dry Ice
• Promoter: CMV

• Gene: Beta-Gal (lacZ), Luciferase
• Cloning Method: Restriction Enzyme ⁄ MCS
• Delivery Type: Transfection
• Selection Agent (Eukaryotic): Puromycin
• Vector: pMIR
• Product Line: Ambion™, pMIR-REPORT™

For Research Use Only. Not for use in diagnostic procedures.