Invitrogen™ Retic Lysate IVT™ Kit

Efficiently translates high molecular weight proteins and both capped and uncapped mRNAs

Brand:  Invitrogen™ AM1200

Product Code. 10137194

Includes: Retic Lysate should be stored at -80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below -70°C. Nuclease-free Water can be stored at any temperature.

Description

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG.

• Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
• Accommodates large volume of template mRNA

How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.

1. Kozak, M. (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18:2828.

Specifications

• Content And Storage: • 5 × 200 μl (store at -80°C)
  • 2 × 1.75 ml Nuclease-free Water (store at any temperature)
  • 100 μl 20X Translation Mix minus-Met
  • 100 μl 20X Translation Mix minus-Leu
  • 100 μl High Salt Mix minus-Met
  • 100 μl Low Salt Mix minus-Met
  • 50 μl Capped Xef-1 RNA (50 μg/ml)
  
  Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
• Expressed Protein Type: Water Soluble Protein
• Expression System: Cell-Free
• No. of Reactions: 60 Reactions
• Product Type: Retic Lysate
• Shipping Condition: Dry Ice

• Format: Kit
• Expression Mechanism: Cell-Free Expression
• For Use With (Application): In Vitro Transcription
• Product Line: Ambion™
• Quantity: 60 Reactions

For Research Use Only. Not for use in diagnostic procedures.