Invitrogen™ RETROscript™ Reverse Transcription Kit

Includes reagents for reverse transcription of RNA into double-stranded cDNA products

Brand:  Invitrogen™ AM1710

Product Code. 10585595

Includes:

• 50μL Control Template RNA (0.5 mg/ml)
• 200μL 10X RT Buffer
• 400μL 10X PCR Buffer
• 300μL dNTP mix (2.5 mM each dNTP)
• 80μL Random Decamers (50 μM)
• 80μL Oligo(dT) Primers (50μL)
• 40μL MMLV-RT (100 units/μL)
• 40μL RNase Inhibitor (10 units/μL)
• 50μL Control PCR Primers (5 μM each)
• 1.0mL High Resolution Gel Loading Solution (5X)
• 3.5mL Nuclease-free Water. 40 reactions.

Description

• Amplify specific RNA targets from complex mixtures (i.e., total cellular RNA)
• Both random decamers and oligo(dT)₁₈ primers provided
• Perform either one- or two-step RT-PCR
• Optimal reaction temperature: 44° to 55°C
• Final product size: 7kb or less

Accessory products: SuperTaq Polymerase (Mfr. Nos. AM2054 and AM2056) and SuperTaq Plus Polymerase (Mfr. Nos. AM2054 and AM2056) are recommended for use with the RETROscript Kit. RETROscript Kit components that are available separately include dNTPS for cDNA Probe Synthesis (Mfr. No. AM8200), Oligo (dT) Primer (Mfr. No. AM5730G), and Random Decamers (Mfr. No. AM5722G). Also available are the T7-Oligo (dT)₂₄ Promoter Primer (Mfr. No. AM5710) and the T7-Oligo (dT)₂₄ Promoter Primer, Anchored (Mfr. No. AM5712).

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Dry ice

Specifications

• Content And Storage: 50μL Control Template RNA (0.5mg/mL); 200μL 10X RT Buffer; 400μL 10X PCR Buffer; 300μL dNTP mix (2.5mM each dNTP); 80μL Random Decamers (50μM); 80μL Oligo(dT) Primers (50μL); 40μL MMLV-RT (100 units/μL); 40μL RNase Inhibitor (10 units/μL); 50μL Control PCR Primers (5μM each); 1.0mL High Resolution Gel Loading Solution (5X); 3.5mL Nuclease-free Water. Store Control Template RNA below -70deg.C. Store all other reagents at -20deg.C.
• Quantity: 40 reactions
• Sample Type: RNA

• Format: Separate Components
• For Use With (Application): cDNA Synthesis, RT-PCR

For Research Use Only. Not for use in diagnostic procedures.