Invitrogen™ RNAqueous™-96 Automated Kit

The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

Brand:  Invitrogen™ AM1812

Product Code. 10249584

Includes: 4 Filter Plates, 4 Collection Plate with lids, 4 Culture Plates, 1 Heat Plate with lid, 100mL Nuclease-free Water, 100mL Elution Solution, 100mL Lysis/Binding Solution, 100mL Rebinding Mix, and 2 x 175mL Wash Solution Concentrate, (1.25mL DNase I (20U/μL) and 12mL DNase 1 Buffer

Description

The Invitrogen RNAqueous-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications.

Specifications

• Content And Storage: • 4 Filter Plates; store at room temperature
  • 4 Collection Plates with lids; room temperature
  • 4 Culture Plates; room temperature
  • 1 Heat Plate with Lid; room temperature
  • 100 mL Nuclease-free Water; room temperature
  • 100 mL Elution Solution; room temperature
  • 100 mL Lysis/Binding Solution; 4°C
  • 100 mL Rebinding Mix; 4°C
  • 2 x 175 mL Wash Solution (concentrated); 4°C
  • 1.25 mL DNase 1; -20°C
  • 12 mL DNase 1 Buffer; -20°C
• Elution Volume: 105 μL
• Final Product Type: Total RNA
• For Use With (Equipment): MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems
• Quantity: 384 Preps
• Starting Material Amount: Cells: ≤10⁵
  Tissue: 0.1–1 mg

• Isolation Technology: Vacuum Filter Plate (Glass Fiber Filter)
• Sample Type: Cells, Tissue
• High-throughput Compatibility: High-throughput Compatible
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Shipping Condition: Box 1: Dry Ice
  Box 2: Room Temperature

For Research Use Only. Not for use in diagnostic procedures.