Invitrogen™ Ambion™ T4 Polynucleotide Kinase (cloned) 10U/μL

Catalyzes the transfer of the terminal phosphate of ATP to 5' hydroxyl termini of DNA or RNA

Brand:  Invitrogen™ AM2310

Product Code. 10697695

Includes: T4 Polynucleotide Kinase

Description

• T4 PNK (PNK, E.C. 2.7.1.78) is recommended for 5' end-labeling of oligonucleotides and polynucleotides
• It also has a 3' phosphatase activity. One tube containing 500U (10U/μL) is provided. T4 Polynucleotide Kinase is rigorously tested for contaminating nonspecific endonuclease, exonuclease, RNase and protease activity, and for functionality in a KinaseMax Kit (Mfr. No. AM1520)
• Reaction buffer is not included with this enzyme
• Unit Definition: One unit is defined as the amount of enzyme catalyzing the production of 1nmol of phosphate to the 5'-OH end of an oligonucleotide from [γ-³²P]ATP in 30 minutes at 37°C

Cloning, DNA & RNA Purification & Analysis, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, Restriction Enzyme Cloning

Order Info

Shipping Condition: Dry ice

For Research Use Only. Not for use in diagnostic procedures.