Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl₂

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

Brand:  Applied Biosystems™ N8080249

Product Code. 10478254

Description

AmpliTaq Gold DNA Polymerase with Buffer II and MgCl₂ is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.

Features

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer II with MgCl₂
• Hot-start technology reduces risk of biological contamination

Notes

• For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
• When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Specifications

• Content And Storage: • AmpliTaq Gold DNA Polymerase (5 U/μL), 5 x 200 μL
  • 10X PCR Buffer II, 20 x 1.5 mL
  • 25 mM MgCl₂, 20 x 1.5 mL
  
  Store at -15°C to -30°C.
• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Dry Ice
• Concentration: 5 U/μL
• Hot Start: Built-In Hot Start
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Format: Tube
• Polymerase: AmpliTaq Gold DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 5,000 units
• For Use With (Application): Hot-start PCR
• Fidelity (vs. Taq): 1X
• No. of Reactions: 4000 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.