CD19 Mouse anti-Human, BD IMag-DM, Clone: HIB19, BD

Mouse Monoclonal Antibody

Brand:  BD Biosciences 551520

Product Code. 15876678

Description

BD IMag™ anti-human CD19 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD19-bearing leukocytes using the BD IMagnet™. CD19 is expressed during all stages of B-cell differentiation and maturation, except plasma cells. CD19 is also present on follicular dendritic cells. It is not found on T cells or on normal granulocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ anti-human CD19 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated twice to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.

Specifications

• Antigen: CD19
• Classification: Monoclonal
• Conjugate: BD IMag-DM
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: HIB19
• Description: B4; B-lymphocyte antigen CD19; Leu-12
• Host Species: Mouse
• Quantity: 10 mL
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C

For Research Use Only.