Invitrogen™ DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA191878BTIN

Product Code. 15212938

Description

MA1-91878-BTIN has been successfully used for Western blot, IF and IP applications. similar epitope as the FLAG tag from Sigma.

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

Specifications

• Antigen: DYKDDDDK Tag
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with proprietary stabilizer and 0.02% sodium azide
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: FG4R
• Conjugate: Biotin
• Gene Alias: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
• Immunogen: Synthetic peptide (DYKDDDDK) coupled to KLH.
• Quantity: 50 μL
• Primary or Secondary: Primary
• Content And Storage: 4°C, do not freeze
• Form: Liquid
• Target Molecule: Tag