Thermo Scientific™ ApaI (10 U/μL)

Cut at GGGCC^C sites with ApaI restriction enzyme, which functions best at 37°C in B buffer (Isoschizomers: PspOMI).

Brand:  Thermo Scientific™ ER1411

Product Code. 10130690

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality – stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP

Note

Incubation at 30°C results in a 2-fold increase in activity. ApaI is inhibited by salt concentrations above 50 mM. ApaI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163.

Specifications

• Concentration: 10 U/μL
• Incubator Temperature: 37°C
• Product Type: Apal

• Components: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
• Quantity: 3000 U

For Research Use Only. Not for use in diagnostic procedures.