Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl₂ and 360 GC Enhancer

The Applied Biosystems™ AmpliTaq™ 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs.

Brand:  Applied Biosystems™ 4398848

Product Code. 10489014

Includes: AmpliTaq 360 Buffer kit is supplied 1.5mL of each 10X AmpliTaq™ 360 Buffer, 25mM M2Cl₂, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl₂, and 360 GC Enhancer is sufficient for use with 250U of enzyme.. All solutions are prepared in deionized, filter-sterilized water.

Description

The Applied Biosystems™ AmpliTaq™ 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs. When used together with or without the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.

• Optimized for the broadest range of targets—from everyday to challenging
• Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
• Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets

Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold™ 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).

As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Note: See user's manual or package insert for limited label license, and trademark information.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Content And Storage: • 10X AmpliTaq 360 Buffer, 1.5 mL
  • 25 mM MgCl₂, 1.5 mL
  • 360 GC Enhancer, 1.5 mL
  
  Store at -15°C to -25°C.
  Product is guaranteed up to 12 months as indicated by the expiration date.
• Polymerase: AmpliTaq 360 DNA Polymerase
• Green Features: Sustainable packaging
• Shipping Condition: Room Temp or Wet Ice
• Concentration: 5 U/μL
• Volume: 1.5 mL

• GC-Rich PCR Performance: High
• Reaction Speed: Standard
• Product Type: GC Enhancer
• For Use With (Application): Standard PCR
• Reaction Format: Kit

For Research Use Only. Not for use in diagnostic procedures.