Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)

Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

Brand:  Applied Biosystems™ 4371435

Product Code. 10096224

Includes: Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I & II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.

Description

The Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.

This easy-to-use kit enables users to calibrate the StepOnePlus Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standards' spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

• Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Order Info

Dry ice shipping; store at -15° to -25°C; protect from light

Specifications

• Content And Storage: Includes one background plate and two 96-well fast reaction plates containing spectra dyes (plates I and II). Plate I contains FAM, VIC, NED, and ROX; Plate II contains SYBR Green I, JOE, and TAMRA.
  
  Store at -15°C to -25°C. Protect from light.
• For Use With (Equipment): Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode
• Product Line: StepOnePlus™
• For Use With (Application): Real Time PCR (qPCR)
• Quantity: 1 Kit

• Calibrated Dye: FAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green
• Detection Method: Fluorescence
• Product Type: Spectral Calibration Kit
• Shipping Condition: Dry Ice
• Calibration Type: Spectral Calibration

For Research Use Only. Not for use in diagnostic procedures.