STEMCELL Technologies ArciTect™ tracrRNA Kit

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

Brand:  STEMCELL Technologies 76016

Product Code. 17189301

Description

• ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing
• ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation
• It is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation
• It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency
• The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA
• ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2 nmol (Catalog #76010), 10 nmol (Catalog #76011), and 20 nmol (Catalog #76012) sizes
• ArciTect™ Cas9 Nuclease is also available separately (Catalog #76001)

Specifications

• Cell Type: Pluripotent Stem Cells
• Quantity: 5 nmol Kit