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Invitrogen™ ARES™ Alexa Fluor™ 647 DNA Labeling Kit
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Quantity:
1 kit
Unit Size:
1 set
Description
The ARES™ Alexa Fluor™ DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor™ 647 dye. The labeled probes can be used for fluorescence in situ hybridization (FISH) and microarray techniques. We offer ARES™ Alexa Fluor™ DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5μg DNA each.
ARES™ Alexa Fluor™ Labeling Kit Specifications:
Dye (Ex/Em): Alexa Fluor™ 647 (650/670 nm)
Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides
Typically produces one dye per 12–20 bases
Optimal for FISH and microarrays
More Uniform Labeling With ARES™ Labeling Kits
ARES™ Alexa Fluor™ Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our FISH Tag™ DNA and FISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.
More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
ARES™ Alexa Fluor™ Labeling Kit Specifications:
More Uniform Labeling With ARES™ Labeling Kits
ARES™ Alexa Fluor™ Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in our FISH Tag™ DNA and FISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.
More Options for Nucleic Acid Labeling
To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Specifications
Specifications
| Content And Storage | Store in freezer (-5 to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| Format | Kit |
| Label or Dye | Alexa Fluor 647 |
| Final Product Type | Probes (Labeled DNA), cDNA (Labeled) |
| Labeling Target | DNA (General), cDNA |
| Includes Label or Dye | Yes |
| Labeling Method | Indirect Labeling |
| Product Line | ARES, Alexa Fluor |
| Product Type | DNA Labeling Kit |
| Show More |
For Research Use Only. Not for use in diagnostic procedures.
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