BAP1 Monoclonal Antibody (1G8), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA146087

Product Code. 11574273

Description

Suggested positive control: HeLa whole cell lysate, antigen standard for BAP1 (transient overexpression lysate).

BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.

Specifications

• Antigen: BAP1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: tris glycine with 0.15M NaCl and 0.05% sodium azide
• Gene Accession No.: Q92560
• Gene Symbols: BAP1
• Immunogen: Human BAP1 protein expressed and purified from E. coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Western Blot
• Clone: 1G8
• Conjugate: Unconjugated
• Gene: BAP1
• Gene Alias: 2300006C11Rik; AA989761; AW553466; BAP1; Brca1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; HUCEP-13; hucep-6; KIAA0272; mKIAA0272; Ubiquitin carboxyl-terminal hydrolase BAP1; ubiquitin C-terminal hydrolase X4; UCHL2; uch-x4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 8314
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid