Gibco™ Basal Medium Eagle (BME)

Basal Medium Eagle (BME)

Brand:  Gibco™ 41010026

Product Code. 11514476

Description

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. Gibco™ BME has since been used for other human lines, including WI-38 and MRC-5.

Gibco™ BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO₂ incubator.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law

cGMP Manufacturing and Quality System
Gibco™ BME is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ BME product made in our Grand Island facility (21010-046). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Basal Medium Eagle (BME), 500mL, Liquid, Phenol Red Indicator, Low Glucose, 12 Months Shelf Life, Store at 2 to 8°C, Protect from light, For Mammalian Cell Culture
• Product Type: Basal Medium
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate
• Quantity: 500 mL

• Content And Storage: 2 to 8°C
• Form: Liquid
• With Additives: Phenol Red
• For Use With (Application): Mammalian cell culture