BD Pharmingen™ IHC Zinc Fixative

An alternative to formalin fixation of tissue for paraffin embedded sections

Brand:  BD 552658

Product Code. 15886838

Description

Ten percent neutral buffered formalin fixes tissue by cross-linking proteins, potentially causing a conformational change or “masking” of the antigenic epitope and leading to reduced or absent antibody reactivity. Different antigen retrieval techniques are used to unmask the epitope; however, many times it is not possible to retrieve the epitope and get optimal immunohistochemical staining. In these situations the use of IHC Zinc fixative, which is a milder fixative than formalin, is recommended.

• Zinc-fixed paraffin embedded sections show very good immunostaining with many leukocytes markers and depict tissue morphology comparable to formalin-fixation
• Use of antigen retrieval techniques on the zinc-fixed paraffin sections may further enhance the antibody reactivity in some cases

There are a few antigens that are not preserved by zinc-fixation. In these situations use of fresh frozen tissue for immunohistochemical staining is the only alternative.

Specifications

• Boiling Point: 100°C
• Content And Storage: Room temperature
• Product Type: Fixative
• For Use With (Application): Immunohistochemical staining
• Form: Liquid

• Color: Clear
• Dilution: 10 X
• Quantity: 500mL
• pH: 7.2

Safety and Handling

Product Identifier

• 10X Zinc Fixative (Formalin Free)

Signal Word

• Danger

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 2
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : zinc acetate, zinc chloride