BD Pharmingen™ IHC Zinc Fixative

An alternative to formalin fixation of tissue for paraffin embedded sections

Product Code	Brand	Dilution	Quantity
10400275	BD 550523	1 X	1000mL
15886838	BD 552658	10 X	500mL

Description

Ten percent neutral buffered formalin fixes tissue by cross-linking proteins, potentially causing a conformational change or “masking” of the antigenic epitope and leading to reduced or absent antibody reactivity. Different antigen retrieval techniques are used to unmask the epitope; however, many times it is not possible to retrieve the epitope and get optimal immunohistochemical staining. In these situations the use of IHC Zinc fixative, which is a milder fixative than formalin, is recommended.

• Zinc-fixed paraffin embedded sections show very good immunostaining with many leukocytes markers and depict tissue morphology comparable to formalin-fixation
• Use of antigen retrieval techniques on the zinc-fixed paraffin sections may further enhance the antibody reactivity in some cases

There are a few antigens that are not preserved by zinc-fixation. In these situations use of fresh frozen tissue for immunohistochemical staining is the only alternative.

Specifications

• Color: Clear
• Product Type: Fixative
• Form: Liquid

• Content And Storage: Room temperature
• For Use With (Application): Immunohistochemical staining