Thermo Scientific™ beta-Galactosidase Assay Stop Solution

The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

Brand:  Thermo Scientific™ 75706

Product Code. 11517830

Description

The Thermo Scientific Mammalian β-Galactosidase Assay Stop Solution is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity.

The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. Alternatively, the included Thermo Scientific M-PER Mammalian Protein Extraction Reagent may be used to prepare lysate from washed adherent or pelleted cells, and then a portion of the lysate used to quantitate the β-Gal activity. With M-PER Reagent, there is no need to scrape cells or to perform freeze-thaw cycles. The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification.

Features of the Thermo Scientific Mammalian β-Galactosidase Assay Kit (assay reagent and stop solution):

• Choose stand-alone assay reagent or complete kit for cell lysis and detection
• Colorimetric assay easily measured in a plate reader or spectrophotometer (405 nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatiible with prepared lysates from other species such as bacteria

Related Products

• Mammalian beta-Galactosidase Assay Kit (Cat. No. 75707)
• beta-Galactosidase Assay Reagent (Cat. No. 75705)

Specifications

• Description: beta-Galactosidase Assay Stop Solution, 25mL, 11.05pH, Liquid, Clear, 96-Well Plate, Reporter Enzyme Assay Type, Colorimetric Detection Method, Mammalian Compatible Cells, Proprietary Substrate, Beta-Gal Substrate, Absorbance Technique
• Formulation: Liquid
• Detection Method: Reporter Enzyme
• Sample Volume: 25mL
• Product Type: β-Galactosidase Assay Stop Solution, beta-Galactosidase Assay Stop Solution
• Format: 96-well Plate
• Product Line: Pierce™
• Substrate Type: Beta-Gal Substrate

• Content And Storage: Store at room temperature.
• For Use With (Application): Protein biology, enzyme reporter assays, protein assays and analysis, beta-galactosidase assays, Absorbance
• pH Range: 11.05
• For Use With (Equipment): Microplate Reader
• Dye Type: Enzyme Labeled
• Quantity: 25mL, 25 mL
• Substrate: Proprietary
• Target: Beta-Galactosidase

For Research Use Only. Not for use in diagnostic procedures.