Eurofins DiscoverX Blk Protein, Active

Active, recombinant full-length mouse Blk, containing an N-terminal His6-tag, for use in Kinase Assays.

Brand:  Eurofins DiscoverX 14-316M

Product Code. 17390124

Description

Activation of B cells by various ligands is accompanied by activation of src-family protein tyrosine kinases. Cross-linking of the B-cell receptor leads to the activation of src-family PTKs BLK (B-cell lymphocyte kinase), Fyn, and Lyn. Specific B and T cell progenitor subsets are preferentially susceptible to transformation by BLK and suggest a role for BLK in the control of proliferation during B cell development. BLK protein tyrosine kinase is found primarily in hematopoietic cells and inhibitors may be of therapeutic use in the treatment of B cell lymphoma.

GenBank M30903. The recombinant protein contains one residue which is in conflict with M30903. This is V421Eand is reported in ESTs BF731187 and BG145526.

Specifications

• Formulation: mg/mL of 50 mM Tris/HCl pH 7.5, 0.1 mM EGTA, 150 mM NaCl, 1 mM benzamidine, 0.03% Brij-35, 0.2 mM PMSF, 0.1% 2-mercaptoethanol, 270 mM sucrose. Frozen solution.
• Quantity: 250 μg
• Recombinant: Recombinant
• Expression System: Recombinant enzyme expressed in Sf21 insect cells
• Purity or Quality Grade: ≥67%

• Molecular Weight (g/mol): 57.7 kDa
• Species: Mouse
• Protein Tag: N-terminal 6His
• Protein Form: Full-length
• Protein: Blk (mouse), active