Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 12%, 1.0 mm, WedgeWell™ format

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.

Product Code	Brand	Wells
15364594	Invitrogen™ NW00120BOX	10-well
15374594	Invitrogen™ NW00122BOX	12-well
15304604	Invitrogen™ NW00127BOX	17-well

Description

Invitrogen Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Similar to NuPAGE Bis-Tris gels, Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.

Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats. Each box contains 10 gels. A Mini Gel Tank (A25977) is required for use of all Bolt gels.

Features include:
• Optimized run conditions—separate your proteins using constant voltage in approximately 20 minutes
• Long shelf life—store gels for at least 16 months at 4–25°C
• Better protein integrity—neutral pH sample preparation process preserves your proteins
• Wedge-shaped wells—for easier sample loading and up to two times higher sample volume

Choose the right Bolt Bis-Tris Plus gels for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. Bolt Bis-Tris Plus gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and 4–12% gradient. The gels can be run using Bolt MES SDS (B000202) or Bolt MOPS SDS (B000102) running buffer to obtain different separation ranges. Bolt Bis-Tris Plus gels consist of a neutral pH-buffered (pH 6.4) polyacrylamide gel and have an operating pH of about 7.0. Bolt Bis-Tris Plus gels also come in four different well formats: 10, 12, 15, and 17 wells.

Convenient to use
Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 20 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

For transferring your proteins to a membrane, we recommend using for Bolt Transfer buffer (BT0006) for traditional wet transfer using the Mini Blot Module (B1000). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001) .

Specifications

• For Use With (Equipment): Mini Gel Tank, XCell SureLock Mini-Cell
• Gel Size: Mini
• Gel Type: Bis-Tris Plus
• Separation Range: 3.5 to 80 kDa
• Mode of Separation: Molecular Weight
• Quantity: 10 Gels/Box
• Storage Requirements: Store at 4–25°C. Do not freeze.
• Width (Metric): 8 cm

• Gel Percentage: 12%
• Gel Thickness: 1.0 mm
• Recommended Applications: Denaturing
• Separation Type: Denaturing
• Product Line: Bolt™, WedgeWell™
• Shipping Condition: Room Temperature
• Length (Metric): 8 cm

Safety and Handling

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