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Invitrogen™ BRAK/CXCL14 Human ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ EHCXCL14X5
Description
The Human BRAK/CXCL14 (BRAK) ELISA quantitates Hu BRAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BRAK. Principle of the method The Human BRAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.Specifications
O95715 | |
700 pg/mL | |
Solid-phase sandwich ELISA | |
ELISA Kit | |
Plasma, Serum, Supernatant | |
5 x 96 assays | |
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
9547 | |
<12% | |
Standard, SAV-HRP, Biotinylated Detection Antibody, Chromogen, Stop Solution, Assay Diluent Concentrate, Pre-coated 96 well plates, Adhesive Plate Covers, Wash Buffer | |
5 x 96 Tests | |
Inflammation | |
2°C to 8°C | |
Human | |
4 hr. 45 min. |
1053 pg/mL to 12,000 pg/mL | |
700 pg/mL to 21000 pg/mL | |
Biotin | |
Chemokine & Receptors | |
This ELISA pair antibody detects human CXCL14. Other species not determined. | |
Human | |
Colorimetric Microplate Reader | |
CXCL14 | |
<10% | |
HRP | |
RUO | |
Plasma, 33 μ;L; Serum, 33 μ;L; Supernatant, 100 μ;L | |
UNQ240/PRO273, BMAC, BRAK, KEC, KS1, MIP-2 g, MIP2G, NJAC, SCYB14 | |
1 hr. 20 min. |