Macherey-Nagel™ Carrier RNA

Carrier-RNA is added to the sample lysate (20 ng/sample) when using compatible NucleoSpin kits (RNA Virus, RNA XS) for optimal performance and highest sensitivity in downstream applications.

Brand:  Macherey-Nagel™ 740514

Product Code. 15361017

Description

For use with RNA isolation using various NucleoSpin and NucleoMag kits: Virus, XS (extra small samples)

• (poly(-A) RNA: poly(A) potassium salt—prepared from ADP with polynucleotide phosphorylase)
• To prepare working solution, dissolve freeze dried Carrier-RNA in lysis buffer (e.g. RA1, RAV1)
• Add to sample lysate (20 ng/sample)
  • Such small amounts typically do not interfere with subsequent RT-PCR, even in oligo-dT primed reverse transcriptions
  • The small amount of Carrier RNA transfered into a reverse transcription reaction does not significantly influence the outcome of the reaction, due to the large excess of oligo-dT primer
• The benefit of adding Carrier RNA depends on sample type, amount, and kind of downstream RNA analysis
• Carrier RNA should be omitted when, subsequent to RNA isolation, a poly-A RNA isolation / RNA sequencing is performed

Specifications

• CAS: 26763-19-6
• Color: Colorless
• Formulation: poly(-A) RNA: poly(A) potassium salt
• For Use With (Application): RNA isolation for downstream applications
• Quantity: 0.3 mg
• Form: Lyophilized

• Concentration: 90 to <100%
• Content And Storage: Refrigerated (4 °C)
• For Use With (Equipment): NucleoSpin Virus and XS kits
• Odor: Odorless
• Sample Source: Prepared from ADP with polynucleotide phosphorylase