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CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), Alexa Fluor™ 488, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 53-1009-42
Description
Description: The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients. Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and B cell. CD100 can also exist as a soluble form. Applications Reported: This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio133-1C6 (133-1C6) antibody has been pre-titrated and tested by flow cytometric of human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.Specifications
CD100 (SEMA4D) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Q92854 | |
Sema4d | |
Affinity chromatography | |
RUO | |
10507 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
eBio133-1C6 (133-1C6) | |
Alexa Fluor 488 | |
Sema4d | |
A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4D; semaphorin H; semaphorin-4D; Semaphorin-C-like 2; semaphorin-J; Semcl2 | |
Mouse | |
100 Tests | |
Primary | |
Human | |
Antibody | |
IgM |
For Research Use Only.