CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen 25-1548-42

Product Code. 15538496

Description

Description: The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activation and induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154. Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat.

CD154 (CD40 Ligand) Monoclonal antibody specifically detects CD154 (CD40 Ligand) in Cynomolgus Monkey, Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD154 (CD40 Ligand)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P29965
• Gene Symbols: CD40LG
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 102138630, 959
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 24-31
• Conjugate: PE-Cyanine7
• Gene: CD40LG
• Gene Alias: CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Cynomolgus Monkey, Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.