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CD278 (ICOS) Monoclonal Antibody (7E.17G9), Biotin, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Invitrogen 13-9942-81
Description
Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells. Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis. Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A-stimulated (3 day) mouse splenocytes and can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.
CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.Specifications
CD278 (ICOS) | |
Monoclonal | |
0.5 mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
Q9WVS0 | |
Icos | |
Affinity chromatography | |
RUO | |
54167 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
7E.17G9 | |
Biotin | |
Icos | |
AILIM, CD278, CVID1, activation-inducible lymphocyte immunomediatory molecule, inducible T-cell costimulator, inducible costimulator | |
Rat | |
50 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2b κ |
For Research Use Only.