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CD279 (PD-1) Monoclonal Antibody (RMP1-14), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
£86.00 - £1428.00
Specifications
Antigen | CD279 (PD-1) |
---|---|
Clone | RMP1-14 |
Concentration | 1 mg/mL |
Applications | Flow Cytometry, Functional Assay |
Classification | Monoclonal |
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
15278847
|
Invitrogen
16-9982-81 |
50 μg | |||||||
15288847
|
Invitrogen
16-9982-85 |
500 μg |
£272.00
500µg |
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15577836
|
Invitrogen
16-9982-38 |
5 mg |
£1428.00
5mg |
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Description
Description: This antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Similar to J43, another mAb to mouse PD-1 (Cat. No. 16-9985), RMP1-14 blocks the binding of both B7-H1-Ig and B7-DC-Ig to PD-1 transfectants. Applications Reported: The RMP1-14 antibody has been reported for use in flow cytometric analysis. Applications Tested: The RMP1-14 antibody has been tested by flow cytometric analysis of 3 day Con A activated splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment.
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "e;danger signals"e; such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.Specifications
CD279 (PD-1) | |
1 mg/mL | |
Monoclonal | |
Liquid | |
RUO | |
PBS with no preservative; pH 7.2 | |
18566 | |
IgG2a κ | |
Affinity chromatography | |
Antibody |
RMP1-14 | |
Flow Cytometry, Functional Assay | |
Functional Grade | |
Rat | |
Mouse | |
Q02242 | |
Pdcd1 | |
Primary | |
4° C | |
Pdcd1 |
For Research Use Only.