IBA Lifesciences CD3 Fab-TACS™ Gravity Kit, Mouse

For manual positive cell separation. The Fab-TACS™ Gravity Kit provides label-free target cells without the use of high affinity antibodies and magnetic beads

Product Code	Brand	Quantity
16265350	IBA Lifesciences 6-3404-004	4 Columns
16275350	IBA Lifesciences 6-3404-010	10 Columns

Description

The kit allows the selection of CD3+ T cells from mouse spleen.

Fab-TACS™ Gravity column (Capacity: 1x108target cells each)

CD3 Fab-Strep, mouse

Buffer CI (10x)

Biotin stock solution

Fab-TACS™ Gravity Adapter

The Fab-TACS™ Gravity column is filled with a Strep-Tactin™ coated matrix made of cell-grade agarose.

Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix.

Subsequently, single-cell tissue suspensions pass through the column.

Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell.

Non-target cells are efficiently washed away.

In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.

After elution, the Fab-Streps self-dissociate from the cell surfaces.

The label-free authentic target cells are now ready for further downstream applications.

Specifications

• Content And Storage: 2°C to 8°C
• Type: Non-magnetic Cell Isolation Kit
• Species: Mouse
• Sufficient For: 1x10⁸ target cells/column