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CD355 (CRTAM) Monoclonal Antibody (Cr24.1), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Brand: Invitrogen 25-3559-41
Description
This monoclonal antibody Cr24.1 reacts with human CD355, also known as Class-I MHC-restricted T-cell-associated molecule (CRTAM). CD355 usually exists as a homodimer, however, it forms a heterodimer with its ligand Necl-2. CD355 is transiently expressed by activated NK, NKT, gamma delta T, and CD8+ T cells. CD355 is also expressed by Purkinje neurons. Human CD355 is involved in immunosurveillance of cancer cells, gamma delta T cell survival, cell polarity, retention of lymphocytes in lymph nodes, promotion of cytotoxicity in NK cells, and IFN gamma secretion from CD8+ T cells. Cr24.1 has been reported to have antagonist activity. This Cr24.1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Filtration: 0.2 μm post-manufacturing filtered. The CRTAM gene is upregulated in CD4-positive and CD8-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains.Specifications
CD355 (CRTAM) | |
Monoclonal | |
5 μL/Test | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide | |
O95727 | |
CRTAM | |
Affinity chromatography | |
RUO | |
56253 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
Cr24.1 | |
PE-Cyanine7 | |
CRTAM | |
CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; class-I MHC-restricted T-cell-associated molecule; cytotoxic and regulatory T cell molecule | |
Mouse | |
25 Tests | |
Primary | |
Human | |
Antibody | |
IgG2a κ |
For Research Use Only.